HCG

Also known as · Human chorionic gonadotropin · Pregnyl · Novarel

01 / Dosing schedule

Cited dosing

The FDA-approved schedule for hypogonadotropic hypogonadism in males is 500–1,000 USP units three times weekly for three weeks, then twice weekly for three weeks. The Coviello 2005 dose-response trial established that 250 IU every other day preserves intratesticular testosterone in men on suppressive TRT.

Pregnyl — male hypogonadotropic hypogonadism

PhaseDose & frequency
Weeks 1–3500–1,000 IU IM three times weekly
Weeks 4–6Same dose reduced to twice weekly
Alternate schedule4,000 IU IM three times weekly for 6–9 months, then 2,000 IU IM three times weekly for 3 months
Route (label)Intramuscular only

Source · FDA label — Pregnyl (2023) ↗

TRT-adjunct — intratesticular-testosterone preservation

PhaseDose & frequency
125 IU every other day−25% change in intratesticular testosterone vs baseline
250 IU every other day−7% — near-eugonadal preservation
500 IU every other day+26% — supraphysiologic
Route in trialSubcutaneous

Source · Coviello AD et al. JCEM 2005 — low-dose hCG maintenance of ITT ↗

Source indication · Selected cases of hypogonadotropic hypogonadism in males (Pregnyl label).

Injection frequency
Two to three times weekly (or every other day for TRT adjunct).
Injection sites
IM: gluteal or deltoid muscle (label). Subcutaneous is used in TRT-adjunct practice.

02 / Reconstitution

Reconstitution reference

Pregnyl is supplied as 10,000 USP units of sterile dried powder in a multi-dose vial with a diluent. Reconstitute the entire diluent volume into the powder vial and agitate gently. Reconstituted solution is stable for 60 days refrigerated. At 10,000 IU in 10 mL diluent = 1,000 IU/mL: a 500 IU dose is 0.5 mL = 50 units on a U‑100 syringe.

Vial10,000 IU
BAC water10 mL (supplied diluent)
Concentration1,000 IU/mL
Example dose500 IU
On a U‑100 syringe50 units on U‑100
Open the reconstitution calculator →

03 / Overview

Protocol overview

HCG is a heterodimeric glycoprotein hormone that binds the LH receptor. In men it stimulates Leydig-cell testosterone production; in women it triggers ovulation and supports the corpus luteum.

The FDA-labeled hypogonadism schedule is decades old and reflects standalone HCG monotherapy. Modern TRT-adjunct practice uses lower every-other-day doses derived from the Coviello 2005 dose-response trial.

Coviello and colleagues established that 250 IU every other day nearly fully preserves intratesticular testosterone in men on 200 mg weekly testosterone enanthate. This is the reference dose-response for the fertility-preservation goal.

The Pregnyl label lists intramuscular administration only; subcutaneous injection is common in current TRT practice and was the route used in the Coviello study.

04 / References

References

  1. FDA prescribing information — Pregnyl (chorionic gonadotropin) 2023 ↗
  2. Coviello AD et al. Low-Dose Human Chorionic Gonadotropin Maintains Intratesticular Testosterone in Normal Men With Testosterone-Induced Gonadotropin Suppression. JCEM 2005 ↗